Application of metagenomic next-generation sequencing in the diagnosis and treatment guidance of Pneumocystis jirovecii pneumonia in renal transplant recipients

Pneumocystis jirovecii pneumonia (PJP) is difficult to be diagnosed, so this study explored if PJP could diagnosed by metagenomic next-generation sequencing (mNGS) and mNGS guide the therapy of PJP. was successfully 13 out 14 recipients with 11 through peripheral blood samples, verified PCR. Ten non-PJP were enrolled as control group. Blood tests revealed a high β-D-glucan (BDG) level in all during hospitalization. Four (28.6%) patients infected cytomegalovirus simultaneously, while 8 (57.1%) suffered from combined infection caused Torque teno virus. Five (35.7%) cases died or subsequent bacteremias/bacterial longer interval between onset diagnosis of/the available against than survival cases. Univariate analysis characteristics that BDG assays higher at admission group (P =0.011). This present supports value detection sample diagnosing PJP, which assist clinical decision for PJ improve outcome The also highlights sensitivity assays. Cytomegalovirus virus infections often occur same time thus can alerts